สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Detection and typing of human papillomavirus DNA in penile carcinoma by polymerase chain reaction

Authors : Nuanchawee Wetprasit*, Sukvirach S**, Kruavon Balachandra*


Affililations:
 
      *National Institute of Health, Department of Medical Sciences,

Bangkok,Thailand

**Research Division, National Cancer Institute, Department of Medical

 Survices, Bangkok, Thailand

           

Source:            Bulletin of Department of Medical Services 1998; 23(10): 628-633

           

Language:         Thai with English abstract

 

Abstract:

 

Specific types of human papillomaviruses (HPV) have been found to implicate as the causative agents in the development of human genital cancer. Penile carcinoma (PC) is a relatively common tumor but the investigation of HPV in PC is still few. In this study, paraffin-embedded tissues of 20 PC from Thai patients were used for detection and typing of HPV infection by polymerase chain reaction and hybridization with specific-type HPV amplified products (HPV 16, 18 and 58). HPV DNA was found in 14 cases (70%) of PC and 6 cases (30%) gave negative results. HPV 18 was the predominant type (65%) whereas HPV 16 and 58 were found in 35% and 15% respectively. Among the 14 positive cases, 5 cases had multiple infections of HPV 16 and HPV 18 while 2 cases had multiple infections of HPV 16, HPV 18 and HPV 58. Identification of HPV  type in PC of Thai patients is useful for further epidemiological study of HPV and genital cancer in Thailand.