สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Authors : Natteewan Poonwan*, Tamae Imai**, Nanthawan Mekha*, Katsukiyo Yazawa**, Yuzuru Mikami**, Akikazu Ando***, Yoshiho Nagata***


Affililations:    *Department of Medical Sciences, National Institute of Health,

Nonthaburi, Thailand

**Research Center for Phatogenic  Fungi and Microbial Toxicoses, Chiba University, Chuo-ku, Chiba (260-8673)

***Department of Bioresources, Chiba University, Matsudo,

Chiba(271), Japan

           

Source:            Journal of Clinical Microbiology 1998; 36(10): 3073-3076  

           

Language:         English

 

Abstract:

 

Thirteen strains of Histoplasma capsulatum were isolated from clinical specimens, including those from AIDS patients, in Thailand. Random amplified polymorphic DNA (RAPD) analysis with three different PCR primers showed that the DNA fingerprint patterns of the Thai isolates were very similar to each other and homogeneous, with only one exceptional strain, although the patterns were clearly different from those of a reference North American strain with all primers tested. Although the difference in the DNA fingerprinting patterns was minor, Thai isolates could be classified into two to four groups. A common PCR band (about 700 bp)in the patterns of all H. capsulatum strains was extracted, and its DNA sequence was determined. A new PCR primer set for the identification of H. capsulatum spicies was developed based on this sequence information. This primer set was 100% successful in the identification of the reference strain as well as all Thai isolates. The results of specificity tests of the primer set for the identification of the fungus are also discussed.