สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Authors : Noppavon Janejai*, Jaranee Pheungphosop*, Wanpen Boonwanich*, Wiyada Charoensiriwatana*


Affililations:
  
     *National Institute of Health, Department of Medical Sciences

           

Source:               Bulletin of Department of Medical Sciences 1998; 40(3): 239-246

 

Language:         Thai with English abstract

 

Abstract:

 

A pilot study for the production of anti-TSH monoclonal antibody has been carried out using 2 Bioreactor systems, i.e. Technomouse; a ready made system and In house Bioreactor which is selfassemble using kidney dialysis cartridage. The hybridoma cells used are able to produce IqG1 upto 2 mg/ml in mouse ascites. The cells are cultured in Bioreactor system using RPMI 1640 enriched with 4.5% glucose. The Technomouse can produce antibody up to 1.3 mg/ml and the total yield is 81 mg while the In house Bioreactor can produce the antibody up to 0.3 mg/ml and the total yield is 141 mg. The consumable cost of the latter system is 3.3 times lower than the former one. Thus, it is possible to develop the Bioreactor system to obtain better efficiency and the system will replace the monoclonal antibody production using experimental animal in a near future.