สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Production and characterization of monoclonal antibody against hepatitis B virus surface antigen

Authors : Nongluk Buddhirakkul*, Chaivat Kittigul**, Prayute Buddhirakkul*, Kruavon Balachandra*


Affililations:       *
National Institute of Health, Department of Medical Sciences

                        **Faculty of Sciences, Kasetsart University

           

Source:               Bulletin of Department of Medical Sciences 1998; 40(2): 127-131

           

Language:         Thai with English abstract

 

Abstract:

 

Specific monoclonal antibodies to Hepatitis B surface Antigen (HBsAg) were produced by fusion of P3-X63-Ag8.653 BALB/c mouse myeloma cells with splenocytes from mice immunized with hepatitis B vaccine. Screening of the monoclonal antibodies were done by Indirect ELISA test. Clones producing the highest anti-HBs titres were used for preparing monoclonal antibodies in mouse ascitic fluid. The monoclonal antibodies in ascitic fluid have titre as high as 1:320,000 Four of monoclonal antibodies, M1-M4, Identified as IgG1 subclass, were tested for their potential useability in development of ELISA kit. The monoclonal antibodies M3 and M4 have specificity to the determinant “a” which should be suitable for use in ELISA test for HBsAg detection.The antibodies M1and M2 have specificity to the determinant “r”and may be used for discrimination of hepatitis B virus subtype.