สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Authors : Natteewan Poonwan*,Yuzaru Mikami*, Suwan Poosuwan* Jotica Boon-Long*, Nanthawan Mekha*, Mayura Kusum*, Katsukiyo Yazawa**, Reiko Tanaka**, Kazuko Nishimura**, Kazuichi Konyama***

Affililations:       *Mycology Section, Division of Clinical Pathology, Department of

Medical Sciences, National Institute of Health, Nonthaburi, Thailand

**Research Center for Pathogenic Fungi and Microbial Toxicoses,

Department of Experimental Chemotherapy, Chiba University,

Chiba, Japan

***JICA (AIDS Project), National Institute of Health,

Nonthaburi,Thailand

 

Source:           Europern Journal of Epidemiology 1997; 13: 335-340

           

Language:         English

 

Abstract:

 

One hundred and thirty-nine strains of Cryptococcus neoformans were isolated in Thailand  from clinical specimens including 97 AIDS patients : 67 from Northern, 48 from Central, 17 from Northeastern and 7 from  Southern regional hospitals. Six out of the 139 strains were serotype B and the remaining 133 were A. There was no correlation between serotypes and regional distribution. To our knowledge, this is the first report of serotyping studies on C. neoformans in Thailand. Studies on random amplified  polymorphic DNA (RAPD) analysis showed that this method is useful for the  differentiation of                  C. neoformans  var. gattii (serotypes B and C) and C. neoformans var. neoformans (serotypes A and D). They also indicated that Thai isolates of C. neoformans var. gattii (serotype B) were a homogeneous group on the basis of their genotypes, Antifungal susceptibility tests using      5 antifungal agents including amphotericin B. fluconazole, flucytosine, itraconazole and micronazole against 50 selected strains of                  C. neoformans  showed that they were sensitive to all of the antifungal agents tested except for one strain that was resistant to flucytosine.