สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

NATIONAL INSTITUTE OF HEALTH OF THAILAND

Nucliotide sequences of the genes coding for the middle surface protein from hepatitis B virus genomes subtypes adr and adw isolated in Thailand

Authors : Duanthanorm Thawaranantha*, Kruavon Balachandra*, Chuenchit Boonchird**, Chedsadaporn Pituksuteepong*, Jittaporn Watanaseree***, Somsak Pantuwatana****

Affililations:    *National Institute of Health, Department of Medical Sciences,

Nonthaburi, 11000

**Department of Biotechnology, Faculty of Science, Mahidol

University

***Government Pharmaceuticals Oganization

****Department of Microbiology, Faculty of Science, Mahidol

University, Bangkok 10400, Thailand      

           

Source:            Journal of the Science Society of  Thailand 1997; 23:  87-100

           

Language:        English

 

Abstract:

 

Viral genomes of 8 isolates of hepatitis B virus (HBV) derived from blood specimens were used to seguence of pre-S2 and S regions. Four samples of each subtype adr and adw  were used. It was found that there was quite high homogeneity among four samples of the same subtype, that is, 99.6% among samples of adr subtype and 98.7% among samples of adw subtype. Although, the two subtypes showed many nucleotide variations, all of them presented the pattern of wild type DNA. Results showed that the isolated DNA of subtype adw could be classified as genotype B whereas those of subtype adr were classified as genotype C. Concerning the most common antigenic determinant a, no variation as vaccine induced escape mutant was observed in all eight samples, for example, at amino acids 126 and 145 of HBsAg.