สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Development of enzyme-linked immunosorbent assay (ELISA) for detection of anti-hepatitis B virus surface antigen

Authors : Panudda Dhepakson*, Kruavon Balachandra*, Kasama Supanaranond*,Narin Hoisanka*

Affililations:       *Nation Institute of Health, Department of Medical Sciences

           

Source:               Bulletin of Department of Medical Sciences 1997; 39(3): 173-178

           

Language:         Thai with English abstract

 

Abstract:

 

The test for detection of anti-hepatitis B virus surface antigen is widely used, the amount of imported test kits are increasing. But the cost still being expensive. Therefore, a sandwich enzyme-linked immunosorbent assay (ELISA) was developed by using HBsAg purified from plasmas of hepatitis B virus carriers as starting materials. Comparison with imported test kit (Sorin Biomedica, Italy), the result showed that 138 and 135 samples were positive by developed ELISA and Sorin Biomedica, respectively and 133 of same samples are positive by both ELISA tests, sensitivity and specificity are 98.5% and 98.1% respectively and the developed ELISA is practical, rapid and high potential in production of diagnotic test kit for anti-HBs detection.