สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Authors : Duanthanorm Thawaranantha*, Kruavon Balachandra* Suttichoke Jongtrakulsiri*, Wanphen Yamkunthong* Kanittha Chimatra**, Jakkriss Bhumiswasdi*

Authors:           Duanthanorm Thawaranantha*, Kruavon Balachandra*

Suttichoke Jongtrakulsiri*, Wanphen Yamkunthong*

Kanittha Chimatra**,  Jakkriss Bhumiswasdi*

 

Affiliations:       *Department of Medical Sciences, Ministry of Public Health,

Nonthaburi, 11000, Thailand

 **Charoenkrungpracharak Metropolis Hospital, Bangkok, Thailand   

 

Source:            Southeast Asian Journal of Tropical Medicine and  Public Health

1995; 26(4): 677-683     

        

Language:       English

 

Abstract:

The DNAs of 17 isolates of varicella-zoster virus (VZV) obtained from 17 Thai individuals with normal varicella or zoster infections (no underlying diseases) were compared by restriction endonuclease analysis using BglI, PstI, EcoRI, Smal and BamHI. The DNA of the Japanese strain, Kawaguchi, was also conducted as a reference DNA. All of virus isolates were confirmed for existence of VZV and VZV-DNA by immunofluorescent test and DNA-hybridization, respectively. Almost all of the Thai epidemiologically unrelated isolates and the Kawaguchi strain could be individually differentiated using BglI, PstI and EcoRI. The other two isolates were identical in restriction profiles even after five endonuclease digestions which Smal and BamHI were the two more enzymes used, therefore, they could be discriminated totally into 16 strains from overall 17 isolates. These findings demonstrate the strain variation of wild-type varicella zoster viruses isolated in Thailand.