สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

NATIONAL INSTITUTE OF HEALTH OF THAILAND

Identification of Blood Meal of Field Caught Aedes aegypti (L.) by Multiplex PCR

Authors : Padet Siriyasatien1, Theerakamol Pengsakul2, Veerayuth Kittichai3, Atchara Phumee3, Sakchai Kaewsaitiam1, Usavadee Thavara2, Apiwat Tawatsin2, Preecha Asavadachanukorn4, Mir S Mulla5

 

Affiliations : 

l Department of Parasitology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
2 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand
3 Medical Science Program, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand
4 Department of Statistic, Faculty of Commerce and Accountancy, Chulalongkorn University, Bangkok, Thailand
5 Department of Entomology, University of California, Riverside, California, USA

Source :

Southeast Asain J Trop Med Public Health, 2010; 41 (1): 43-47

Language : English

Abstract :

 
 

        Laboratory bred female Aedes aegypti (L.) was used to determine sensitivity of multiplex PCR for detecting human blood meal. Human blood DNA was detected in live fully fed mosquitoes until 3 days after blood feeding, and for 4 weeks when stored at -20 C. Among 890 field caught female mosquito samples examined for vertebrate DNA by multiplex PCR, results were positive for human, pig, dog, cow and mixture of 2 host DNA at 86.1, 3.4, 2.1, 1.0 and 3.6%, respectively, while 3.9of the samples were negative. Blood feeding pattern must be considered when mosquito control strategies become employed.