สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Development of Lethal Ovitrap with Natural Attractants to Control DHF Vectors

Authors : U. Thavara1, A. Tawatsin1, J. Chompoosri1, P. Bhakdeenuan1, P. Kunhachan1, S. Sangkitporn1, P. Siriyasatien2,3

 

Affiliations : 

1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand,
2Chulalongkorn University, Faculty of Medicine, Department of Parasitology, Bangkok, Thailand,
3Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand          

 

Source : The 14th Conference of the International Society of Travel Medicine 24-28 May 2015, Quebec City ,Canada
Language : English 
 

Abstract :

 
 

        Ovitraps are artificial breeding containers attracting gravid female mosquitoes to lay eggs and they have been used for surveillance of dengue haemorrhagic fever (DHF) and chikungunya vectors for years; however, these ovitraps could be also modified as tools for integrated vector control. To obtain an effective lethal ovitrap, evaluations of attractants to Aedes aegypti (L.) and Ae. albopictus Skuse to ovitraps treated with larvicides were then carried out under laboratory and field conditions. To obtain candidate oviposition attaractants, we used a water rinse of 3 mollusk species: blood cockle (Anadara granosa), carpet shell (Paphia undulata), sea mussel (Mytilus smaragdinus), and the giant tiger prawn (Penaeus monodon) compared with tap water. The rinse water of carpet shell and giant tiger prawn showed higher attractiveness for oviposition than the other candidate attractants. Regarding larvicidal comparison, the mean number of eggs laid in ovitraps containing temephos and Bacillus thuringiensis israelensis (Bti) were significantly higher than those laid in ovitraps containing novaluron and untreated ovitraps. No ovicidal effect of the larvicides used in ovitraps was observed since hatching of eggs occurred; however, all larvae died eventually. Attempts have been also made to identify the most essential attractive parts of the rinse water of carpet shell and giant tiger prawn for further commercial synthetic attractants. In field experiments, evaluations of lethal ovitraps having rinse water of carpet shell and giant tiger prawn as attractants treated with temephos compared with the untreated ovitraps were carried out in rubber plantation habitats in Trang province, southern Thailand. There was no significant difference between the mean number of eggs laid in the lethal ovitraps and those laid in the untreated ovitraps. This clearly indicated that no repellent effect was observed in the lethal ovitraps. The lethal ovitrap obtained from this study could possibly be used as an effective, economical and simple mean of DHF vector control.