สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Authors : U. Thavara1, S. Sunantaraporn2, V. Sanprasert3, T. Pengsakul4, A. Phumee3, R. Boonserm3, A. Tawatsin1, P. Siriyasatien3,5

 

Affiliations : 

1 National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand,
2 Chulalongkorn University, Medical Science Program, Faculty of Medicine, Bangkok, Thailand,
3 Chulalongkorn University, Department of Parasitology, Faculty of Medicine, Bangkok, Thailand,
4 Prince of Songkla University, Faculty of Medical Technology, Songkhla, Thailand,
5 Excellence Center for Emerging Infectious Diseases, King Chulalongkorn Memorial Hospital, Thai Red Cross Society, Bangkok, Thailand

          

Source : The 14th Conference of the International Society of Travel Medicine 24-28 May 2015, Quebec City ,Canada
Language : English 
 

Abstract :

 
 

        Head lice infestation is spread throughout the world, the disease is caused by Pediculus humanus capitis. By the advent of molecular techniques, head lice have been classified into three clades. Recent reports demonstrated that pathogenic organisms could be found in head lice. However, information of head lice and its pathogenic bacteria in Thailand have never been investigated. In this study, we determined genetic diversity of head lice collected from various areas of Thailand and demonstrated the presence of Acinetobacter spp. in the head lice. Total DNA was extracted from 275 head louse samples which were collected from several geographic regions of Thailand. PCR was used to amplify the COIgene of head lice and for detection of Bartonella spp. and Acinetobacter spp.. The amplified PCR amplicons were cloned and sequenced. DNA sequences were analyzed by Neighbor-joining method using Kimura's 2 -parameter model. Phylogenetic tree constructed base on COI gene revealed that head lice in Thailand were clearly classified into two clades (A and C). Bartonella spp. was not detected in all samples while Acinetobacter spp. were detected in 10 samples (3.6%) which consisted of A. baumannii(1.45 %), A. radioresistens (1.45 %), and A. schindleri (0.7%). Moreover, the relationship ofAcinetobacter spp. and clade of head lice showed that Acinetobacter spp. was found both on clade A and C. Head lice in Thailand were classified into clade A and B based on the COI gene sequences. Pathogenic Acinetobacter spp. was detected in both clades. Data obtained from the study may assist to develop effective strategies for head lice control in the future. Moreover, detection of pathogenic bacteria in the head lice could raise awareness of head louse as a source for nosocomial bacterial infection.