สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Authors : Surang Tantivanich*, Malinee Chittaganpitch**, Srisin Khunsmith*, Sirima Pattamadilok**


Affilications:       *Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400;

                        **Department of Medical Sciences, National Institute of Health, Nonthaburi 11000, Thailand
           
Source:             Southeast Asian Journal of Tropical Medicine and Public Health 
1996; 27(4)
                       
Language:         English
 
Abstract:
 
Thirty-one hybridomas producing monoclonal antibodies (MAbs) against structural proteins of RSV subgroup A (Long strain) and RSV subgroup B (Japanese wild strain) were produced and separated into three groups by their reactivities with RVS-A and RVS-B using IFA. Group I was specific to RSV-A , group II was specific to RSV-B and GROUP III was specific to both subgroups. Characterization of selected two Mabs from each group indicated that three Mabs recognized phosphoproteins (P) and the others recognized fusion protein (F).All of the selected Mabs were IgG1 and carried kappa light chain. Those selected Mabs can be used to detect the presence of RSV from NPAs and classify them into two subgroup. The infection rates of RSV in Thai children are very low and most of them were RSV subgroup A.