สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Subacute toxicity study of tradition medicinal Trikatuk

Authors : Pranee Chavalittumrong*, Aimmanas Attawish*, Pat Rugsamon*, Pranee Chuntapet**


Affiliations:   
     *Division of Medicinal Plant Research and Development

**Division of Clinical Pathology, Department of Medical Sciences
 
Source:             Bulletin of Department of Medical Sciences 1996; 38(4): 273-292.
 
Language:         Thai with English abstract
 
Abstract:
 
            Trikatuk, a preparation of Thai traditional medicine used to adjust patient’s element during rainy season is composed of three constituents, namely Zingiber officinale rhizomes (ginger), Piper nigrum fruits (papper) and Piper retrofractum fruits (long pepper). Three formulae of Trikatuk, i.e. Pitta, Wata and Semha. Containing different ratios of each herbal component are used in rainy season for the treatment of illness due to fire, wind and water element, respectively. Pitta formula contains 12 parts of ginger, 8 parts of pepper and 4 parts of long pepper, while Wata formula consists of 4 parts of ginger, 12 parts of pepper and 8 parts of long pepper and Semha formula is composed of 8 parts of ginger, 4 parts of pepper and 12 parts of long pepper.
            Subacute toxicity studies of water extracts of the three formulae of Trikatuk were conducted in adult Wistar rats. The extracts were administered orally once daily for ten consecutive days at the doses equivalent to 0.36, 2.52 and 17.64 g of crude drug/kg body weight/day which are equivalent to 1, 7 and 49 folds of therapeutic dose, respectively. The changes of certain hematological parameters found in some extract-treated groups were not likely to be caused by the extracts, and were within normal values. The results of biochemical studies of the serum samples indicated that the groups receiving the extract of Pitta formula and Semha formula had significantly higher levels of albumin and total proteins than the controls. Similarly, the group treated with high dose of Wata formula extract also had higher albumin and total protein levels. The increase of serum albumin in the animals treated with the extracts may be the result of elevated albumin synthesis in the liver. Histopathological examinations of the liver and kidney tissue specimens showed no differences in pathological findings between the extract-treated groups and the control groups.