สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Authors : Payu Bhakdeenuan *, Kanok Preativatanyou **, Sunchai Payungporn ***, Usavadee Thavara ****, Apiwat Tawatsin ****, Kom Sukontason *****, Kabkaew L. Sukontason *****, Wej Choochote *****, Padet Siriyasatien **
Affiliations:   
 

* Medical Sciences Program, Department of Parasitology, Faculty of Medicine, Chulalongkom University, Thailand
** Department of Parasitology, Faculty of Medicine, Chulalongkom University, Thailand
*** Department of Biochemistry, Faculty of Medicine, Chulalongkom University, Thailand
**** National Institute of Health, Department ofMedical Sciences, Ministry of Public Health, Thailand
***** Department of Parasitology, Faculty of Medicine, Chiang Mai University, Thailand



 
Source:   XXIV International Congress of Entomology August 19-25,2012 Daegu, Korea

 
Language:  
English

 
Abstract:
 

        In order to determine post mortem interval (PM I) using flies collected from corpses more precisely, accurately identification of flies is essential. Morphology identification of fly especially in the same genus is difficult and required highly expert personals. In this study we evaluate the using of molecular techniques for identification forensically important sarcophigid flies in Thailand using ITS2 gene. A total of 26 specimens of four sarcophigid fly species (Sarcophaga dux, S. ruficornis, S. peregrina and S. scopariiformis) collected from various regions of Thailand were used. When compared sequences with database, the percentage identities range from 99 to 100. Intraspecific variation of each species was 0-0.3%, while the maximum interspecific variation was 19.7%. PCR-RFLP was able to differentiate these four sarcophigid species. The results of this study would be valuable for forensic personnel in order to identify species of forensically importance flies collected from the crime scenes more accuracy and therefore accurate estimate of the PMI.

Keyword: Sarcophigid fly, Intemal Transcribed Spacer 2, Molecular identification, PCR-RFLP, Thailand