สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Decrease of mosquito salivary gland proteins after a blood meal: An implication for pathogenesis of mosquito bite allergy

Authors : Padet Siriyasatien*, Kuntida Tangthongchaiwiriya*, Kanyarat Kraivichian*, Surang Nuchprayoon*, Apiwat Tawatsin**, Usavadee Thavara**

 

Affiliations:        * Department of Parasitology, Faculty of Medicine, Chulalongkorn
University, Bangkok 10330, Thailand
** National Institute of Health, Department of Medical Sciences,
Ministry of Public Health, 88/7 Tiwanon Road, Nonthaburi 11000, Thailand
 
Source:             Presented in the 4th International Congress of Vector Ecology (SOVE),Reno, Nevada, U.S.A., 2 – 7 October 2005
 
Language:         English
 
Abstract:
 
Salivary gland protein profiles of Aedes aegypti (L) and Culex quinquefasciatus Say pre- and post-blood feeding were analyzed. SDS-PAGE studies before blood feeding of Ae. aegypti demonstrated 8 major polypeptide bands of 20, 35, 37, 42, 45 47, 70 kDa and a high molecular weight band >118 kDa,whereas those of Cx. quinquefasciatus demonstrated 9 major polypeptide bands of 20, 26, 36, 38, 45, 47, 49 kDa and 2 high molecular weight bands >118 kDa. After a blood feeding, salivary gland polypeptides of Ae. aegypti: 35, 37, 45, 47, 70 kDa and high molecular weight band >118 kDa were depleted, while the polypeptide bands of 20, 26, 36, 38 kDa were depleted in Cx. quinquefasciatus. This study indicates that these major polypeptides were introduced into vertebrate hosts during mosquito took a blood meal. Further investigation in molecular, biochemical and immunological aspects of these salivary gland polypeptides may provide information for better understanding in the role of these proteins in mosquito bite allergy.