สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Authors : Siripakorn Sangkitporn*, Sutudsanee Vimolsarte**, Boonnipa Songkharm***, Khwanchai Wangkahat****, Ativet Savetadul*****, Phanthipa Sriviroonchote******, Amara Yowang*******, Ornchira Supangwiput*, Somchai Sangkitporn*

Affiliations:       *National Institute of Health

                                **Regional Medical Science Center Khonkaen

***Regional Medical Science Center Udonthani
****Regional Medical Science Center Ubonratchathani        
*****Regional Medical Science Center Trang
******Regional Medical Science Center Suratthani
*******Regional Medical Science Center Chiangrai
 
Source:            Journal of Health Science 2005; 14: 24-33
 
Language:        Thai with English abstract
 
Abstract:
 
The accurate and reliable measurement of hemoglobin separation and determination is essential for thalassemia diagnosis. The validation of quality control material KKU-Hb by characterization of their homogeneity and their stability in storage during April to June 2004 was described. HPLC and LPLC instruments were used for hemoglobin separation and Hb A2 determination. Results of homogeneity study showed that KKU-Hb had small coefficient of variation ranging between 1.43-4.58% and 4.27-5.56% for intralaboratory study and interlaboratory study, respectively. These materials could be stored at –6oC for 2 months without significant decrease in Hb A2 level (t-test, p>0.05). In conclusion, the validation, homogeneity and stability of KKU-Hb support their use as quality control material for measurement of hemoglobin separation and determination by HPLC and LPLC instruments.