สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Detection of hepatitis B virus DNA in sera by dot blot hybridization test

Authors : Nuanchawee Wetprasit*, Pikul Haisupasin**, Kruavon Balachandra*, Nongluk Buddhirakkul*, Jakkriss Bhumisawasdi*


Affiliations:        *Health Science Research Institute, Department of Medical Sciences

                        **National Cancer Institute, Department of Medical service                     
Source:              Journal of the Medical Technologist Association of Thailand
1996; 24(1): 35-42                   
Language:         Thai with English abstract
 
Abstract:
In this study, dot blot hybridization test was applied in detection of hepatitis B virus DNA (HBV-DHA) in serum. Extracted HBV-DNA was dotted onto nitrocellulose membranes and hybridized with HBV-DNA probe which was labeled with alkaline phosphatase. The purple colour is developed by using the specific substrate in the reaction. Examined sera were divided into 3 groups (100 cases/group): HBV carrier group (HBsAg), suspected HBV infection group (HBsAg, high level of liver enzymes) and normal group (HBsAg, normal level of liver enzymes). The results showed that HBV-DNA were detected in 22%, 8% and 4% of HBV carrier, suspected and normal group, respectively. This indicated that dot blot hybridization technique had high specificity and sensitivity especially in case of suspected group which was negative by immunodiagnosis. Furthermore, this technique could be applied and very useful for evaluation the efficiency of selective drugs in treatment of patients by consideration of quantitative changing of HBV-DNA in sera.