Affiliations: *Virus Research Institute, Department of Medical Sciences,
National Institute of Health, Nonthaburi,11000, Thailand,
**Department of Hygiene, Sapporo Medical University School of
Medicine, Sapporo 060 Japan
Source: Journal of Clinical Microbiology 1996; 34(5): 1050-1057
A total of 557 fecal specimens collected from piglets with diarrhea in Thailand were examined for rotavirus RNA by polyacrylamide gel electrophoresis. Twenty -three, one, and two samples were positive for group A, group B, and group C rotaviruses, respectively.Two samples exhibited two segments found in picobirnavirus RNA. RNA electropherotyping of 23 group A rotaviruses showed that they were classified into five patterns. By serotyping by enzyme-linked immunosorbent assay and PCR, viruses in 3 and 14 specimens were found to be serotype G3 and serotype G10 , respectively. For one specimens, containing a serotype G10 virus ( strain P343), virus was isolated in MA -104 cells , and the nucleotide sequences of the VP7 and VP4 genes were determined. Comparative sequence analysis and cross-neutralization tests showed that strain P343 has B223-like G10 and UK-like P7 serotypes(or VP4genotype 5) specificities. Rotaviruses having such antigenic specificities have not been detected in piglets. Thus, the interspecies transmission of rotaviruses between cows and pigs was suggested.