สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Serological and genomic characterization of porcine rotaviruses in Thailand: detection of a G10 porcine rotavirus

Authors : Yaowapa Pongsuwanna*,**, Koki Taniguchi1**, Malliga Chiwakul*, Tomoko Urasawa**, Fumiyo Wakasugi**, Chuinrudee Jayavasu*, Shozo Urasawa**

 

Affiliations:       *Virus Research Institute, Department of Medical Sciences,

National Institute of Health, Nonthaburi,11000, Thailand,

**Department of Hygiene, Sapporo Medical University School of

Medicine, Sapporo 060 Japan     

Source:           Journal of Clinical Microbiology 1996; 34(5): 1050-1057    

Language:       English

 

Abstract:

A total of 557 fecal specimens collected from piglets with diarrhea in Thailand were examined for rotavirus RNA by polyacrylamide gel electrophoresis. Twenty -three, one, and   two samples were positive for group A, group B, and group C rotaviruses, respectively.Two samples exhibited two segments found  in picobirnavirus RNA. RNA electropherotyping of 23 group A rotaviruses showed that they were classified into five patterns. By serotyping by enzyme-linked immunosorbent assay and PCR, viruses in 3 and 14 specimens were found to be serotype G3 and serotype G10 , respectively. For one specimens, containing a serotype G10 virus ( strain P343), virus was isolated in MA -104 cells , and  the nucleotide sequences of the VP7 and VP4 genes were determined. Comparative sequence analysis and cross-neutralization tests showed that strain P343 has B223-like G10 and UK-like P7 serotypes(or VP4genotype 5) specificities. Rotaviruses having such antigenic specificities have not been detected in piglets. Thus, the interspecies transmission of rotaviruses between cows and pigs was suggested.