สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Authors : Aroon Bangtrakulnonth* ,Jiroj Sasipreeyajan**, Pathom Sawanpanyalerd***, Sumalee Boonmar****, Nopharat Marnrim*and Chaiwat Pulsrikarn*

 

Affiliations:        *WHO National Salmonellae and ShigellaeCenter, Department of Medical Sciences, Ministry of Public Health, Thailand
**Department of Veterinary Medicine, Faculty of Veterinary Science, Chulalongkorn   University, Henri Dunan Rd., Patumwan, Bangkok 120330. Thailand
***Department of Medical Sciences, Ministry of Public Health, Thailand
****Department of Microbiology and Immunology. Faculty of Veterinary Medicine, Kasetsart University
 
Source:            The Proceedings of 41st Kasetsart University Annual Conference: 481-489
 
Language :        Thai with English abstract
 
Abstract :         
 
One hundred twenty-six broiler chicken intestines were sampled during October 2000 to September 2001 from farms in 8 provinces in the central, eastern and northeastern regions in Thailand that included Bangkok, Ayutthaya, Nakhon pathom, Chonburi, Lopburi, Prachinburi, Chachaengsoa and Nakhonratchasima. Isolation and identification of Yersinia enterocolitica and Salmonella spp. were performed using the methods described in the ISO 10173 and ISO 6579, respectively. Yersinia enterocolitica was not found in all the samples examined. However, Salmonella spp. were identified in 17.46 percent of all the samples (22/126). Salmonella samples were found from only 4 provinces, i.e. Nakhon Pathom, Ayutthaya, Lopburi and Bangkok with the percentages of 33.33, 30.00, 28.57 and 15.00, respectively. All of the Salmonella spp. isolates were verified and categorized into 8 serovars that included S. Enteritidis (8 isolates), S. Blockley (5 isolates), S. enterica subsp. enterica 4,12:-:1,2 (3 isolates), S. Schwarzengrund (2 isolate), S. Agona       (1 isolate), S. Emek (1 isolate), S. Virchow   (1 isolate) and S. Albany (1 isolate). The isolates were further tested for antimicrobial resistance to 9 antimicrobial agents by disk susceptibillty test. The data from the test were as followed: 4.55% (1/22) of isolated were resistant to Cefotaxime, 4.55% (1/22) resistant to Kanamycin, 9.09% (2/22) resistant to Gentamicin, 9.09% (2/22) resistant to Norfloxacin, 22.73% (5/22) resistant to Chloramphenicol, 27.27% (6/22) resistant to Ampicillin, 27.27% (6/22) resistant to Sulfamethoxazole/Trimethoprim, 40.91% (13/22) resistant to Streptomycin and 45.45% (10/22) resistant to Tetracycline.