สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Morphology of 24 Reference Serovars of Leptospira by Electron Microscopy

Authors : Prukswan Chetanachan*, Pimjai Naigowit*, Kruavon Balachandra*, Mayurachat Biaklang*, Pathom Sawanpanyalert*

 

Affiliations :       *National Institute of Health, Department of Medical Sciences

 Source :            Disease Control Journal 2003; 3(7-9): 162-167
 
Language :        Thai with English abstract
 
Abstract :
 
Leptospirosis caused by Leptospira is still a public health problem. The objective of this study was to demonstrate the morphology of 24 reference serovars of Leptospira by transmission electron microscope. We found that all serovars showed helical appearance similar to a rope with 17 – 36 spirals, 0.1 – 0.2 mm in diameter and 10 – 22 mm of length while the end of both sides were bended like a hook. Most of them were medium size, only serovars named copenhageni and saigon were specially long and large while serovars sarmin was rather short and thick. At magnification x 20,000, we could see an axial filament laying thoughout both sides of the body and at magnification x 90,000, the outer membrane sheath covering the whole body appeared clearly. The specific morphology of leptospira investigated by electron microscopic method could be beneficially applied to other aspects of study such as direct observation of the pathogen isolated from patients specimens or investigation of infected tissues.