สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

NATIONAL INSTITUTE OF HEALTH OF THAILAND

Laboratory and field evaluation of novaluron, a new acylurea insect growth regulator, against Aedes aegypti (Diptera: Culicidae)

Authors : Mir S. Mulla*, Usavadee Thavara**, Apiwat Tawatsin**, Jakkrawarn Chompoosri**, Zaim M***, Tianyun Su *

 

Affiliations:        * Department of Entomology, University of California, Riverside, CA 92521, U.S.A
** National Institute of Health, Department of Medical Sciences, Ministry of Public Health, 88/7 Tiwanon Rd., Nonthaburi 11000, Thailand
                        *** WHOPES, World Health Organization, Geneva, Switzerland
 
Source:             Journal of Vector Ecology 2003 Dec; 28(2): 241-54
 
Language:         English
 
Abstract:
 
Novaluron, a new chitin synthesis inhibitor type of insect growth regulator, was evaluated in the laboratory and field against larvae of the mosquito Aedes aegypti (L.). In the laboratory, the technical material showed a high level of activity against 2nd and 4th instar larvae. The inhibition of emergence (IE) was 100% at concentrations of 0.25 to 1.0 microg/L. Second instars were slightly more susceptible than fourth instars. At the high concentration (1 ppb), most of the mortality occurred in the larvae, but at sublethal concentrations (for larvae) mortality also occurred in pupae and adults (incomplete eclosion). An EC10 (emulsifiable concentrate containing 10% active ingredient) formulation of novaluron was evaluated against Ae. aegypti in water-storage containers in the field in Thailand. Two tests at high (0.05 to 1 mg/L) and low (1 to 20 microg/L) concentrations were carried out in clay jars (200 L) and plastic pails (75 L). At high concentrations in clayjars, we obtained 86-96% inhibition of emergence (IE) for about 190 d, but the mortality declined at low concentrations after 190 d. At much lower concentrations, in jars and plastic pails in the second experiment, the IE was 80-100% for 2 mo. The highest concentrations of this series (10 microg/L in jars and 20 microg/L in pails) were active for 75 and 68 d respectively, losing efficacy by 82 d post-treatment. In the experiment using low concentrations, all treatments in jars and plastic pails failed to provide adequate control 90 dafter treatment. These studies show that novaluron, even as EC10, has exceptional long-term activity against Ae. aegypti in water-storage containers, with higher concentrations yielding greater long-term control than low concentrations.