สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

NATIONAL INSTITUTE OF HEALTH OF THAILAND

Validation of automated liquid chromatography system for diagnosis of thalassemias and hemoglobinopathies

Authors : Somchai Sangkitporn*, Parichat Pung-amritt**, Siripakorn K Sangkitporn*, Areerat Sangnoi* and Voravarn S Tanphaichitr**

 

Affiliations:         *National Institute of Health, Department of Medical Sciences, Ministry of PublicHealth, Nonthaburi, Thailand
**Department of Pediatrics, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok, Thailand
 
 
Source:            Southeast Asian Journal of Tropical Medicine and Public Health 2002; 33: 862-868
 
 
Language:        English
 
 
Abstract:
 
Accurate and precise hemoglobin separation and the quantitation of Hb A2 and Hb F are essential for the diagnosis of thalassemias and hemoglobinopathies. Presented in this study is the validation of the Hb A2 assay of HbGold analyzer, a fully automated liquid chromatography system for hemoglobin separation and quantitation. Variability of Hb A2 quantitation was quite low; the CV’s of within run, between run and interlaboratory studies were 1.8-3.1%, 3.4-6.0% and 6.8-8.8%, respectively. Results of %Hb A2 quantitated by HbGold analyzer correlated well with those given by Bio-Rad Variant Hb testing system (r=0.98). The application of HbGold analyzer for diagnosis of thalassemia phenotypes frequently observed in Thailand is considered. In conclusion, Hb A2 assay of HbGold analyzer could be used for the quantitation of Hb A2 and Hb F and the presumptive identification of abnormal hemoglobins.