สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Authors : Suranga Suguanwongse*, Nuanjun Ruchusatsawat*, Lamduan Changlaw**, Suthon Vongsheree*, Paijit Warachit***

 
Affiliations:        *Virus Research Institute, Department of Medical Sciences.

                        **Phayao Hospital, Phayao Province.
                        ***Department of Medical Sciences.       
Source:             Journal of Health Sciences 1996; 5(1): 101-108               
Language:         Thai with English abstract
 
Abstract:
An alternative strategy for HIV-1 seropositive confirmation test was studied using combination of two or three screening tests. Four commercially available screening tests : (Three ELISA tests and one agglutination assay) were evaluated. There were 620 HIV-1 seropositive sera collected from Phayoa Hospital and many hospitals in the central region of Thailand. It was found that all four tests could not reveal 100% accuracy compared to Western blot analysis. There were 9 discrepant samples which were actually low titer of anti-HIV-1 antibodies. Here, we proposed a combination of 2 or 3 screening tests as an alternative confirmatory strategy which could assure not only the positive cases, but also indicate the indeterminate ones. This alternative test might be beneficial to laboratories in regional areas since it offered simpler, more rapid and less expensive protocol for HIV-1 confirmation.