Affiliation: *National Institute of Health, Department of Medical Sciences
Source: Journal of Health Science 2001; 10: 688-694
Language: Thai with English abstract
Dengue virus which causes dengue fever (DF) and dengue hemorrhagic fever (DHF) consists of 4 serotypes, DEN1, DEN2, DEN3 and DEN4. This study was to identify the dengue serotype by RT-PCR. The specimens were from 4 hospitals at 4 provinces of Thailand, Ratchaburi hospital, Hatyai hospital at Songkhla province, Lampang hospital and Maharaj Nakhonratchasima hospital from June 1999 until December 2000. By RT-PCR, in 1999, 192 cases were found positive, of which 48.4, 34.4, 12.5 and 4.7 per cent were affected with DEN2, DEN1, DEN3 and DEN4 respectively. Of the 308 positive cases in 2000, DEN1, DEN2, DEN3 and DEN4 were identified in 153 cases (49.7%), 32 cases (10.4%), 111 cases (36.0%) and 11 cases(3.6%), respectively. Dual infection with DEN1 and DEN3 was found in only one case (0.3%). The dominant DEN1 in 2000 was found in 3 provinces. Although DEN3 was predominant in Ratchaburi, DEN1 was also reportedly high. There was significant correlation between officially reported dengue cases of Ratchaburi province and DEN3 – positive cases, and also DEN1 – positive cases of Ratchaburi hospital (p=0.01). The conclusion was that DEN2 was dominant in 1999 but DEN1 was dominant in 2000. DEN3 and DEN1 might be the main cause of dengue outbreak at Ratchaburi in 2000.