สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Authors : Sumonta Chaisomchit*, Supaporn Nammoonnoy*, Wiyada Charoensiriwatana*

Affiliations:        *National Institute of Health, Department of Medical Sciences,

Tiwanond Road, Muang, Nonthaburi11000

 

Source:             Bulletin of Department of Medical Sciences 2001; 43(1)

 

Language:         Thai with English abstract

 

Abstract:

 

We have presented here an in vitro assay system for screening of anti-hepatitis B virus (HBV) activity. A HBV producing cell line was used as an in vitro system for the test method. The principle of the developed assay system was to directly determine inhibition of HBV replication, i.e. inhibition of HBV DNA production, by the anti-HBV agents, using a dot blot analysis with electrochemiluminescence. The results have shown that our developed assay system could clearly detect the HBV inhibition activity of a reference drug, lamivudine (Glaxo Wellcome, UK) and it could sensitively detect as low as 4.8 x 10-5 pmole of HBV  DNA. The method would, therefore, be appropriately used as a screening method for research in investigation of anti-HBV agents, particularly in medicinal plants.