สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Authors : Areerat Sa-ngasang*, Yauwaman Sutivigit**


Affiliations:        *National Institute of Health, Department of Medical Sciences,

Tiwanond Road, Amphur Maung, Nonthaburi 11000 Tel. 9510000

**Songkhla Regional Medical Sciences Center 616/1 Moo 2

Tumbol Pawong Amphur Maung Songkhla 90100 Tel. (074) 333203-4

           

Source:           Bulletin of Department of Medical Sciences 2000; 42(4): 1-12

 

Language:       Thai with English abstract

 

Abstract:

 

Ten percent of Dengue hemorrhagic fever (DHF) reported cases were sent for serological confirmation at National institute of Health (NIH), Department of Medical Science (DMSCs). During 1987 to 1999, about 7,350 samples/year were tested. Of these, eighty five percent of samples were collected by using Nobuto’s type 1-filter paper, which imported with high cost. In this study, another blood filter collection paper, named Whatman blood filter collection (BFC) 180 was compared. Cutting the paper into the size that could adsorp 100 microliters of blood. During October-November 1998, blood samples from twenty cases (37 samples) of suspected DHF cases from hospital in Songkhla province were collected by both filter-paper Whatman BFC 180 and Nobuto’s type 1, one piece per sample. The entire samples were sent to detect anti-dengue antibody by Hemagglutination inhibition (HI) test and IgM/IgG antibody capture ELISA at NIH, DMSCs. There are no significant difference in serological diagnosis and IgG ELISA unit (p = 0.21 and 0.65, respectively), however considerably low significant difference (p = 0.024) in IgM ELISA unit. Its is likely that there is a chance to use a new filter-paper which has same property but cheaper instead the old one.