Affiliations: *National Institute of Health, Department of Medical Sciences,
Tiwanond Road, Nonthaburi 11000
**Hematology Section, Rajvithi Hospital, Rajvithi Road, Bangkok 10400
Source: Bulletin of Department of Medical Sciences 1999; 41(1): 23-31
Language: Thai with English abstract
The studies on dried blood specimens for hemoglobin A2 (Hb A2 ) separation and determination levels of bb - thalassemia carrier were carried out by fully automated HPLC. Five microlitre of EDTA blood at optimal Hb concentration 11.5 + 1.2 g/dL (Hb A2 > 3.5%) were equivalent to 7 millimetre in diameter of dried blood specimen on filter paper giving an appropriate quantiation area of chromatogram. The results showed that dried blood specimens from B - thalassemia carrier were stable with minimal change of Hb A2 level up to 7 days of storage at room temperature. The handling of dried blood specimen provides several advantages over than usual including decrease risk of infection during collection and transportation of specimens from community hospital.