สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Studies on dired blood specimens for hemoglobin A2 saparation and determination levels of B-thalassemia carrier by high performance liquid chromatography

Authors : Somchai Sangkitporn*, Pattraporn Boonkhant**, Siripakorn Sangkitporn*

Affiliations:        *National Institute of Health, Department of Medical Sciences,

   Tiwanond Road, Nonthaburi 11000         

 **Hematology Section, Rajvithi Hospital, Rajvithi Road, Bangkok 10400

 

Source:               Bulletin of Department of Medical Sciences 1999; 41(1): 23-31

 

Language:         Thai with English abstract

 

Abstract:

 

The studies on dried blood specimens for hemoglobin A2          (Hb A2 ) separation and determination levels of bb - thalassemia carrier were carried out by fully automated HPLC. Five microlitre of EDTA blood at optimal Hb concentration 11.5 + 1.2  g/dL (Hb A2  > 3.5%) were equivalent to 7 millimetre in diameter of dried blood specimen on filter paper giving an appropriate quantiation area of chromatogram. The results showed that dried blood specimens from   B - thalassemia carrier were stable with minimal change of Hb A2  level up to 7 days of storage at room temperature. The handling of dried blood specimen provides several advantages over than usual including decrease risk of infection during collection and transportation of specimens from community hospital.