สถาบันวิจัยวิทยาศาสตร์สาธารณสุข

National Institute of Health of Thailand

Production and characterization of monoclonal antibodies specific to Vibrio cholerae 0139

Authors : Aree Thattiyaphong*, Sriwanna Huttayananont*, Krongkaew Supawat*, Paijit Warachit*

Affiliations:        *National Institute of Health, Department of Medical Sciences,

Tiwanond Road, Nonthaburi 11000

 

Source:             Bulletin of Department of Medical Sciences 1999; 41(2): 103-119

 

Language:        Thai with English abstract

 

Abstract:

 

Four mouse hybridomas producing monoclonal antibodies (MSbs) to <i>Vibrio cholerae</i> O139 antigens, NHV1, NHV2, NHV3, NHV4, were established by hybridoma technique. Mabs produced by NHV1 and NHV3 were of immunoglobulin M (IgM) isotypes; whereas, Mabs from NHV2 and NHV4 were of IgG2a and IgG3 isotypes, respectively. Monoclonal antibodies secreted by NHV1, NHV2, and NHV3, could detect both         <i>V. cholerae</i> O1 and O139 antigens. Monoclonal antibody produced by NHV4, specifically recognized <i>V. cholerae</i> O139 lipopolysaccharide (LPS) Out of 4 hybridoma clones, the NHV4 gave the highest antibody titre, antigenic sensitivity and no cross-reaction was observed against whole cell lysates of <i>V. cholerae</i> O1, non-O1/non O139, other <i>Vibrio</i> spp, as well as other enteric bacteria by indirect ELISA. The antigenic specificity was determined by indirect ELISA and Western Blot analysis. The acquisition of these monoclonal antibodies provide reagents which would be very useful in further development of simple immunodiagnostic assays for diarrhel stool, food and environmental samples.